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GAPDH (基因名), Glyceraldehyde-3-phosphate dehydrogenase (蛋白名), g3p_human.

产品名称:

GAPDH Antibody

货号:

P1932Rb

商标:

EIAab^®

监管等级:

RUO

别名:

Peptidyl-cysteine S-nitrosylase GAPDH, CDABP0047, OK/SW-cl.12, GAPDH, GAPD

序列号:

P04406

反应性:

human,mouse,rat,pig,arabidopsis ,corn

免疫原:

Synthetic peptide

形态:

Liquid

存储说明:

4℃ for frequent use, -20℃ to -70℃ for 6 months

缓冲液:

0.1M×PBS,50% glycerol,pH7.5

浓度:

200ug/ml

纯化方式:

Immunogen affinity purified

克隆性:

Polyclonal

亚型:

Rabbit IgG

应用:

推荐产品:

Goat Anti-Rabbit IgG(H+L) HRP-conjugated Antibody

研究领域:

Neurosciences

规格 & 价格:

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Human GAPDH Polyclonal Antibody

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产品说明书

数据表:

MSDS:

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R&D 技术数据

Western blot analysis of antibody specificity using a routine panel composed of GAPDH depleted human plasma and protein lysates from selected human tissues and cell lines.

Immunohistochemical analysis of human salivary gland cancer, using GAPDH antibody at 1:600 dilution.

通用注释

亚单元:

Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules (By similarity). Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with phosphorylated RPL13A; inhibited by oxidatively-modified low-densitity lipoprotein (LDL(ox)). Component of the GAIT complex. Interacts with FKBP6; leading to inhibit GAPDH catalytic activity.

功能:

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

亚细胞位置:

Cytoplasm Cytosol Nucleus Cytoplasm Perinuclear region Membrane Cytoplasm Cytoskeleton Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

数据库链接

UniGene:

Hs.544577

SMR:

P04406

STRING:

9606.ENSP00000229239

KEGG:

hsa:2597

MIM:

138400

Pfam: