首页 > 重组蛋白 > Human EZR Recombinant Protein

EZR (基因名), Ezrin (蛋白名), ezri_human.

产品名称:

Human EZR/ Ezrin Recombinant Protein
Ezrin

货号:

R1297h

商标:

EIAab^®

监管等级:

RUO

别名:

Cytovillin, Villin-2, p81, VIL2

序列号:

P15311

来源:

E.coli

种属:

Human

标签:

His

序列:

291-415aa

预估分子量:

13.75 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Epigenetics

规格 & 价格:

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Human EZR Protein

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human EZR protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 6q25.3 by HGNC 6q25.3 by Entrez Gene 6q25.3 by Ensembl

EZR Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with MPP5 and SLC9A3R2. Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B (PubMed:9314537, PubMed:12651155, PubMed:15498789, PubMed:17616675, PubMed:17881735, PubMed:19555689). Interacts (when phosphorylated) with FES/FPS (PubMed:18046454). Interacts with dimeric S100P, the interaction may be activating through unmasking of F-actin binding sites (PubMed:12808036, PubMed:19111582). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity). Detected in a complex composed of at least EZR, AHNAK, PPL and PRX.

功能:

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

亚细胞位置:

Apical cell membrane Peripheral membrane protein Cytoplasmic side Cell projection Cell projection Microvillus membrane Peripheral membrane protein Cytoplasmic side Cell projection Ruffle membrane Peripheral membrane protein Cytoplasmic side Cytoplasm Cell cortex Cytoplasm Cytoskeleton Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side).

数据库链接

UniGene:

Hs.487027

SMR:

P15311

STRING:

9606.ENSP00000338934

KEGG:

hsa:7430

MIM:

123900

Pfam:

PF00769

Uniprot:

P15311

相关产品

序列长度:

586

序列:

仅供参考，详细请咨询（services@eiaab.com）

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586:

DIIHNENMRQ | GRDKYKTLRQ | IRQGNTKQRI | DEFEAL

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells."

(PMID: 18270268)

[2].

"Interaction of ezrin with the novel guanine nucleotide exchange factor PLEKHG6 promotes RhoG-dependent apical cytoskeleton rearrangements in epithelial cells."

(PMID: 17881735)

[3].

"Podocalyxin increases the aggressive phenotype of breast and prostate cancer cells in vitro through its interaction with ezrin."

(PMID: 17616675)

[4].

"Ca2+-dependent binding and activation of dormant ezrin by dimeric S100P."

(PMID: 12808036)

[5].

"Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein ezrin."

(PMID: 1382070)

[6].

"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

(PMID: 20628086)

[7].

"Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score."

(PMID: 20379614)

[8].

"Genetic susceptibility to distinct bladder cancer subphenotypes."

(PMID: 19692168)

[9].

"Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"."

(PMID: 20628624)

[10].

"CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity."

(PMID: 20335315)

[1].

人Ezrin(EZR)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人Ezrin(EZR)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人Ezrin(EZR)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人Ezrin(EZR)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人Ezrin(EZR)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人Ezrin(EZR)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人Ezrin(EZR)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人Ezrin(EZR)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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