首页 > 重组蛋白 > Human LCN2 Recombinant Protein

LCN2 (基因名), Neutrophil gelatinase-associated lipocalin (蛋白名), ngal_human.

产品名称:

Human LCN2/ Neutrophil gelatinase-associated lipocalin Recombinant Protein
中性粒细胞明胶酶相关脂质运载蛋白

货号:

R1388h

商标:

EIAab^®

监管等级:

RUO

别名:

25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin, p25, NGAL, HNL, NGAL

序列号:

P80188

来源:

E.coli

种属:

Human

标签:

His

序列:

21-198aa

预估分子量:

19.58 kDa (monomer)

纯度:

Greater than 95% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cardiovascular

规格 & 价格:

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产品说明书

说明书:

MSDS:

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数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

The PCR product of human LCN2 gene was determined by 1% Agarose stained with EB.

Recombinant human LCN2 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 9q34.11 by HGNC 9q34.11 by Entrez Gene 9q34.11 by Ensembl

LCN2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Monomer (PubMed:7683678, PubMed:1281792). Homodimer; disulfide-linked (PubMed:7683678). Heterodimer; disulfide-linked with MMP9 (PubMed:7683678).

功能:

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413, PubMed:27780864, PubMed:20581821). Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:27780864). Can also bind siderophores from M.tuberculosis (PubMed:15642259, PubMed:21978368).

亚细胞位置:

Secreted Cytoplasmic granule lumen Cytoplasmic vesicle lumen Upon binding to the SLC22A17 (24p3R) receptor, it is internalized (By similarity). Releases the bound iron in the acidic lumen of cytoplasmic vesicles (PubMed:12453413, PubMed:20581821).

数据库链接

UniGene:

Hs.204238

SMR:

P80188

STRING:

9606.ENSP00000277480

KEGG:

hsa:3934

MIM:

600181

Pfam:

PF00061

Uniprot:

P80188

相关产品

序列长度:

198

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

201:

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"The changes of serum sKlotho and NGAL levels and their correlation in type 2 diabetes mellitus patients with different stages of urinary albumin"

C Wu, Q Wang, C Lv, et al.

From: Diabetes Research and Clinical Practice

[1].

"Ligand preference inferred from the structure of neutrophil gelatinase associated lipocalin."

(PMID: 10684642)

[2].

"Molecular cloning and expression of a cDNA encoding NGAL: a lipocalin expressed in human neutrophils."

(PMID: 8060329)

[3].

"Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase."

(PMID: 7683678)

[4].

"Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection."

(PMID: 20587610)

[5].

"Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"."

(PMID: 20628624)

[6].

"Neutrophil gelatinase-associated lipocalin and hepcidin: what do they have in common and is there a potential interaction?"

(PMID: 20502037)

[7].

"Neutrophil gelatinase-associated lipocalin (NGAL): a new piece of the anemia puzzle?"

(PMID: 20512104)

[8].

"Plasma neutrophil gelatinase-associated lipocalin and acute postoperative kidney injury in adult cardiac surgical patients."

(PMID: 20435938)

[9].

"NGAL knockdown by siRNA in human cholangiocarcinoma cells suppressed invasion by reducing NGAL/MMP-9 complex formation."

(PMID: 20373132)

[10].

"Neutrophil gelatinase associated lipocalin as a biomarker for acute kidney injury in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass."

(PMID: 20363104)

[1].

人中性粒细胞明胶酶相关脂质运载蛋白(LCN2)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人中性粒细胞明胶酶相关脂质运载蛋白(LCN2)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人中性粒细胞明胶酶相关脂质运载蛋白(LCN2)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人中性粒细胞明胶酶相关脂质运载蛋白(LCN2)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人中性粒细胞明胶酶相关脂质运载蛋白(LCN2)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人中性粒细胞明胶酶相关脂质运载蛋白(LCN2)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人中性粒细胞明胶酶相关脂质运载蛋白(LCN2)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人中性粒细胞明胶酶相关脂质运载蛋白(LCN2)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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