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HMGB1 (基因名), High mobility group protein B1 (蛋白名), HMGB1_HUMAN.

产品名称:

Human HMGB1/ High mobility group protein B1 ELISA Kit
高迁移率族蛋白B1

货号:

E0399h

商标:

EIAab^®

监管等级:

RUO

别名:

High mobility group protein 1, HMG-1, HMG1

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.079ng/mL

特异性:

Natural and recombinant human High mobility group protein B1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Epigenetics

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献 ( 6 )
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤6.8%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤10.1%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	91	85-97
Plasma	93	87-99

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	110-122%	95-104%	85-97%	100-110%
EDTA plasma(n=5)	113-123%	98-106%	103-112%	97-109%
heparin plasma(n=5)	101-109%	93-105%	96-105%	89-101%

通用注释

亚单元:

(Microbial infection) Interacts with adenovirus protein pVII; this interaction immobilizes HMGB1 on chromatin, thus preventing its release from cell and subsequent inflammation activation.

功能:

In the extracellular compartment (following either active secretion or passive release) involved in regulation of the inflammatory response. Fully reduced HGMB1 (which subsequently gets oxidized after release) in association with CXCL12 mediates the recruitment of inflammatory cells during the initial phase of tissue injury; the CXCL12:HMGB1 complex triggers CXCR4 homodimerization (PubMed:22370717). Induces the migration of monocyte-derived immature dendritic cells and seems to regulate adhesive and migratory functions of neutrophils implicating AGER/RAGE and ITGAM (By similarity). Can bind to various types of DNA and RNA including microbial unmethylated CpG-DNA to enhance the innate immune response to nucleic acids. Proposed to act in promiscuous DNA/RNA sensing which cooperates with subsequent discriminative sensing by specific pattern recognition receptors (By similarity). Promotes extracellular DNA-induced AIM2 inflammasome activation implicating AGER/RAGE (PubMed:24971542). Disulfide HMGB1 binds to transmembrane receptors, such as AGER/RAGE, TLR2, TLR4 and probably TREM1, thus activating their signal transduction pathways. Mediates the release of cytokines/chemokines such as TNF, IL-1, IL-6, IL-8, CCL2, CCL3, CCL4 and CXCL10 (PubMed:12765338, PubMed:18354232, PubMed:19264983, PubMed:20547845, PubMed:24474694). Promotes secretion of interferon-gamma by macrophage-stimulated natural killer (NK) cells in concert with other cytokines like IL-2 or IL-12 (PubMed:15607795). TLR4 is proposed to be the primary receptor promoting macrophage activation and signaling through TLR4 seems to implicate LY96/MD-2 (PubMed:20547845). In bacterial LPS- or LTA-mediated inflammatory responses binds to the endotoxins and transfers them to CD14 for signaling to the respective TLR4:LY96 and TLR2 complexes (PubMed:18354232, PubMed:21660935, PubMed:25660311). Contributes to tumor proliferation by association with ACER/RAGE (By similarity). Can bind to IL1-beta and signals through the IL1R1:IL1RAP receptor complex (PubMed:18250463). Binding to class A CpG activates cytokine production in plasmacytoid dendritic cells implicating TLR9, MYD88 and AGER/RAGE and can activate autoreactive B cells. Via HMGB1-containing chromatin immune complexes may also promote B cell responses to endogenous TLR9 ligands through a B-cell receptor (BCR)-dependent and ACER/RAGE-independent mechanism (By similarity). Inhibits phagocytosis of apoptotic cells by macrophages; the function is dependent on poly-ADP-ribosylation and involves binding to phosphatidylserine on the cell surface of apoptotic cells (By similarity). In adaptive immunity may be involved in enhancing immunity through activation of effector T cells and suppression of regulatory T (TReg) cells (PubMed:15944249, PubMed:22473704). In contrast, without implicating effector or regulatory T-cells, required for tumor infiltration and activation of T-cells expressing the lymphotoxin LTA:LTB heterotrimer thus promoting tumor malignant progression (By similarity). Also reported to limit proliferation of T-cells (By similarity). Released HMGB1:nucleosome complexes formed during apoptosis can signal through TLR2 to induce cytokine production (PubMed:19064698). Involved in induction of immunological tolerance by apoptotic cells; its pro-inflammatory activities when released by apoptotic cells are neutralized by reactive oxygen species (ROS)-dependent oxidation specifically on Cys-106 (PubMed:18631454). During macrophage activation by activated lymphocyte-derived self apoptotic DNA (ALD-DNA) promotes recruitment of ALD-DNA to endosomes (By similarity).

亚细胞位置:

Nucleus Chromosome Cytoplasm Secreted Cell membrane Peripheral membrane protein Extracellular side Endosome Endoplasmic reticulum-Golgi intermediate compartment In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune and non-immune cells such as macrophages, monocytes, neutrophils, dendritic cells and natural killer cells in response to various stimuli such as LPS and cytokines involves a nonconventional secretory process via secretory lysosomes (PubMed:12231511, PubMed:14532127, PubMed:15944249). Secreted by plasma cells in response to LPS (By similarity). Found on the surface of activated platelets (PubMed:11154118). An increased chromatin association is observed when associated with the adenovirus protein pVII (PubMed:27362237).

数据库链接

UniGene:

Hs.434102

SMR:

P09429

STRING:

9606.ENSP00000343040

KEGG:

hsa:3146

MIM:

163905

Pfam:

PF00505

Uniprot:

P09429

相关产品

Mouse Hmgb1 ELISA Kit

Rat Hmgb1 ELISA Kit

Bovine HMGB1 ELISA Kit

Chicken HMGB1 ELISA Kit

Pig HMGB1 ELISA Kit

序列长度:

215

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

218:

EEEEDEEDED | EEEDDDDE

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Alarmins (IL-33, sST2, HMGB1, and S100B) as potential biomarkers for schizophrenia"

E Kozłowska, E Brzezińska-Błaszczyk, J Agier, et al.

From: Journal of Psychiatric Research

[2].

"Assessment of HMGB-1 concentration in tick-borne encephalitis and neuroborreliosis"

P Penza, P Czupryna, O Zajkowska, et al.

From: International Journal of Infectious Diseases

[3].

"Is HMGB1 a New Indirect Marker for Revealing Fibrosis in Chronic Hepatitis and a New Therapeutic Target in Treatment?"

A Albayrak, MH Uyanik, S Cerrah, et al.

From: Viral Immunology

[4].

"Elevated serum levels of high mobility group box protein 1 (HMGB1) in patients with ankylosing spondylitis and its association with disease activity.."

P Oktayoglu, S Em, M Tahtasiz, et al.

From: Rheumatology International

[5].

"Effect of tumour necrosis factor-α inhibitor on serum level of dickkopf-1 protein and bone morphogenetic protein-7 in ankylosing spondylitis patients"

M Korkosz, J Gasowski, P Leszczynski

From: Scandinavian Journal of Rheumatology

[6].

"Resveratrol Does Not Benefit Patients with Non-alcoholic Fatty Liver Disease"

VS Chachay, GA Macdonald, JH Martin, et al.

From: Clinical Gastroenterology and Hepatology

[1].

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒可以做多少个样本？

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒使用视频？

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人高迁移率族蛋白B1(HMGB1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人高迁移率族蛋白B1(HMGB1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人高迁移率族蛋白B1(HMGB1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人高迁移率族蛋白B1(HMGB1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human HMGB1/ High mobility group protein B1 ELISA Kit 高迁移率族蛋白B1

E0399h

High mobility group protein 1, HMG-1, HMG1

Human HMGB1/ High mobility group protein B1 ELISA Kit
高迁移率族蛋白B1