首页 > 重组蛋白 > Human AKT2 Recombinant Protein

AKT2 (基因名), RAC-beta serine/threonine-protein kinase (蛋白名), akt2_human.

产品名称:

Human AKT2/ RAC-beta serine/threonine-protein kinase Recombinant Protein
RACβ丝氨酸/苏氨酸蛋白激

货号:

R9360h

商标:

EIAab^®

监管等级:

RUO

别名:

Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, PKB beta

序列号:

P31751

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human AKT2 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 19q13.2 by HGNC 19q13.2 by Entrez Gene 19q13.2 by Ensembl

AKT2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with BTBD10 (By similarity). Interacts with KCTD20 (By similarity). Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CLK2, PBH2 and TRAF6. Interacts (when phosphorylated) with CLIP3, the interaction promotes cell membrane localization (PubMed:19139280). Interacts with WDFY2 (via WD repeats 1-3) (PubMed:16792529).

功能:

One of the few specific substrates of AKT2 identified recently is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during insulin-stimulated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. Phosphorylates CLK2 on 'Thr-343'.

亚细胞位置:

Cytoplasm Nucleus Cell membrane Peripheral membrane protein Early endosome Localizes within both nucleus and cytoplasm of proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. By virtue of the N-terminal PH domain, is recruited to sites of the plasma membrane containing increased PI(3,4,5)P3 or PI(3,4)P2, cell membrane targeting is also facilitared by interaction with CLIP3. Colocalizes with WDFY2 in early endosomes (By similarity).

数据库链接

UniGene:

Hs.631535

SMR:

P31751

STRING:

9606.ENSP00000375892

KEGG:

hsa:208

MIM:

125853

Pfam:

PF00169

Uniprot:

P31751

相关产品

序列长度:

481

序列:

仅供参考，详细请咨询（services@eiaab.com）

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481:

TPPDRYDSLG | LLELDQRTHF | PQFSYSASIR | E

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas."

(PMID: 1409633)

[2].

"MEK1 and AKT2 mutations in Japanese lung cancer."

(PMID: 20354455)

[3].

"Akt2 and Akt3 play a pivotal role in malignant gliomas."

(PMID: 20167810)

[4].

"Genetic variations in the PI3K/PTEN/AKT/mTOR pathway are associated with clinical outcomes in esophageal cancer patients treated with chemoradiotherapy."

(PMID: 19164214)

[5].

"First evidence of genetic association between AKT2 and polycystic ovary syndrome."

(PMID: 18768676)

[6].

"Akt2 Gene common allelic variants in insulin resistance and the metabolic syndrome."

(PMID: 17576055)

[7].

"Analysis of genetic variation in Akt2/PKB-beta in severe insulin resistance, lipodystrophy, type 2 diabetes, and related metabolic phenotypes."

(PMID: 17327441)

[8].

"A family with severe insulin resistance and diabetes due to a mutation in AKT2."

(PMID: 15166380)

[9].

"Akt1 and Akt2: differentiating the aktion."

(PMID: 21432781)

[10].

"Ankrd2/ARPP is a novel Akt2 specific substrate and regulates myogenic differentiation upon cellular exposure to H(2)O(2)."

(PMID: 21737686)

[1].

人RACβ丝氨酸/苏氨酸蛋白激(AKT2)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人RACβ丝氨酸/苏氨酸蛋白激(AKT2)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人RACβ丝氨酸/苏氨酸蛋白激(AKT2)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人RACβ丝氨酸/苏氨酸蛋白激(AKT2)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人RACβ丝氨酸/苏氨酸蛋白激(AKT2)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人RACβ丝氨酸/苏氨酸蛋白激(AKT2)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人RACβ丝氨酸/苏氨酸蛋白激(AKT2)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人RACβ丝氨酸/苏氨酸蛋白激(AKT2)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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